Mouse Complement C3 ELISA Kit (ab157711) (2024)

Overview

• Product name

  Mouse Complement C3 ELISA Kit
  See all C3/C3b kits

• Detection method

  Colorimetric

  See Also

  KeyMander - Keyboard & Mouse Adapter for Game ConsolesMouse Spondin-1 (SPON1) ELISA KitMouse PDGFR beta ELISA Kit (ab253225)Mouse CXCL1 ELISA Kit (GRO alpha) (ab213859)

• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Overall				< 10%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Overall				< 10%

• Sample type

  Serum, Plasma

• Assay type

  Sandwich (quantitative)

• Sensitivity

  1.6578 ng/well

• Range

  3.13 ng/ml - 200 ng/ml

• Recovery

  > 85 %
  

  Sample specific recovery

  Sample type	Average %	Range
  Serum	> 85	% - %

• Assay duration

  Multiple steps standard assay

• Species reactivity

  Reacts with: Mouse
  Predicted to work with: Rat

• Product overview

  Mouse Complement C3 ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Complement C3 in mouse biological samples.

  
  

  In this assay the Complement C3 present in samples reacts with the anti- Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti- Complement C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

• Platform

  Microplate

Properties

• Storage instructions

  Store at +4°C. Please refer to protocols.

• Components	1 x 96 tests
  100X HRP-conjugated anti-mouse Complement C3 antibody	1 x 150µl
  20X Wash Buffer Concentrate	1 x 50ml
  5X Diluent Concentrate	1 x 50ml
  Chromogen Substrate Solution	1 x 12ml
  Mouse Complement C3 Calibrator (lyophilized)	1 vial
  Mouse Complement C3 ELISA Microplate	1 unit
  Stop Solution	1 x 12ml

• Research areas

  • Immunology
  • Innate Immunity
  • Complement
  • Classical Pathway

  • Immunology
  • Innate Immunity
  • Complement
  • Alternative Pathway

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Synapse marker

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Complement ELISA kits

• Function

  C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
  Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

• Tissue specificity

  Plasma.

  See Also

  Mouse CXCL1 ELISA Kit (GRO alpha) (ab216951)

• Involvement in disease

  Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
  Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.

• Sequence similarities

  Contains 1 anaphylatoxin-like domain.
  Contains 1 NTR domain.

• Post-translational
  modifications

  C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
  Phosphorylation sites are present in the extracelllular medium.

• Cellular localization

  Secreted.

• Information by UniProt

• Alternative names

  • Acylation stimulating protein cleavage product
  • AHUS5
  • ARMD9

  see all

• Database links

  • Entrez Gene: 12266 Mouse
  • Entrez Gene: 24232 Rat
  • SwissProt: P01027 Mouse
  • SwissProt: P01026 Rat
  • Unigene: 19131 Mouse
  • Unigene: 11378 Rat

• Sample results

  Complement C3measured in biological fluids of healthy speciesshowing quantity (ng) per mL of tested sample. Samples were diluted 8000-128000 fold.

• Standard Curve

  Representative standard curve using ab157711 Complement C3 Mouse ELISA kit.

• Protocol Booklet

Click here to view the general protocols

• SDS download

  Country/Region

  Language

  

• Datasheet download

  Download

ab157711 has been referenced in 26 publications.

• Varshney R et al. Neonatal intake of Omega-3 fatty acids enhances lipid oxidation in adipocyte precursors. iScience 26:105750 (2023). PubMed: 36590177
• Mehdipour M et al. Small-animal blood exchange is an emerging approach for systemic aging research. Nat Protoc 17:2469-2493 (2022). PubMed: 35986217
• Kennedy EM et al. Development of intravenously administered synthetic RNA virus immunotherapy for the treatment of cancer. Nat Commun 13:5907 (2022). PubMed: 36207308
• Jung BJ et al. Lipoteichoic Acid from Staphylococcus aureus Activates the Complement System via C3 Induction and CD55 Inhibition. Microorganisms 9:N/A (2021). PubMed: 34074052
• Mei B et al. Dexmedetomidine attenuates sepsis-associated inflammation and encephalopathy via central α2A adrenoceptor. Brain Behav Immun 91:296-314 (2021). PubMed: 33039659

View all Publications for this product