Mouse PDGFR beta ELISA Kit (ab253225) (2024)

Overview

• Product name

  Mouse PDGFR beta ELISA Kit
  See all PDGFR beta kits

• Detection method

  Colorimetric

• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Serum	8			3.3%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Serum	3			2.6%

• Sample type

  Cell culture supernatant, Serum, Cell culture media, EDTA Plasma

  See Also

  KeyMander - Keyboard & Mouse Adapter for Game ConsolesMouse Spondin-1 (SPON1) ELISA KitMouse Complement C3 ELISA Kit (ab157711)Mouse CXCL1 ELISA Kit (GRO alpha) (ab213859)

• Assay type

  Sandwich (quantitative)

• Sensitivity

  20.3 pg/ml

• Range

  109.4 pg/ml - 7000 pg/ml

• Recovery

  Sample specific recovery

  Sample type	Average %	Range
  Cell culture supernatant	105	104% - 107%
  Serum	93	90% - 95%
  Cell culture media	104	103% - 106%
  EDTA Plasma	112	106% - 116%

• Assay time

  1h 30m

• Assay duration

  One step assay

• Species reactivity

  Reacts with: Mouse
  Does not react with: Cow

• Product overview

  Mouse PDGFR beta ELISA Kit (ab253225) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGFR beta protein in cell culture media, cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PDGFR beta with 20.3 pg/ml sensitivity.

  
  

  SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  
  

  - Single-wash protocol reduces assay time to 90 minutes or less
  - High sensitivity, specificity and reproducibility from superior antibodies
  - Fully validated in biological samples
  - 96-wells plate breakable into 12 x 8 wells strips

  
  

  A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

• Notes

  PDGFR beta (Platelet-derived growth factor receptor beta) is a tyrosine kinase receptor for members of the platelet derived growth factor family, hom*odimeric PDGFB and PDGFD, and heterodimeric PDGFA/B. Binding plays an essential role in embryonic development, blood vessel development, and cell proliferation, migration, survival, chemotaxis, and differentiation.

• Platform

  Pre-coated microplate (12 x 8 well strips)

Properties

• Storage instructions

  Store at +4°C. Please refer to protocols.

• Components	1 x 96 tests
  10X Mouse PDGFR beta Capture Antibody	1 x 600µl
  10X Mouse PDGFR beta Detector Antibody	1 x 600µl
  10X Wash Buffer PT (ab206977)	1 x 20ml
  Antibody Diluent 4BI	1 x 6ml
  Mouse PDGFR beta Lyophilized Recombinant Protein	2 vials
  Plate Seals	1 unit
  Sample Diluent NS (ab193972)	1 x 50ml
  SimpleStep Pre-Coated 96-Well Microplate (ab206978)	1 unit
  Stop Solution	1 x 12ml
  TMBDevelopment Solution	1 x 12ml

• Research areas

  • Cardiovascular
  • Angiogenesis
  • Growth Factors
  • Other

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • PDGF

  • Microbiology
  • Organism
  • Virus
  • RNA Virus
  • ssRNA positive strand virus
  • SARS Coronavirus

  • Cancer
  • Growth factors
  • PDGF

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factor receptors

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Neuroscience
  • Development

• Function

  Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.

• Involvement in disease

  Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
  Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
  Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
  Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
  Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.

• Sequence similarities

  Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
  Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  Contains 1 protein kinase domain.

• Post-translational
  modifications

  Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.

• Cellular localization

  Membrane.

• Information by UniProt

• Alternative names

  • Beta platelet derived growth factor receptor
  • Beta-type platelet-derived growth factor receptor
  • CD 140B

  see all

  See Also

  Mouse CXCL1 ELISA Kit (GRO alpha) (ab216951)

• Database links

  • Entrez Gene: 18596 Mouse
  • SwissProt: P05622 Mouse
  • Unigene: 4146 Mouse

• SimpleStep ELISA Protocol Diagram

  SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

• Example of mouse PDGFR beta standard curve in Sample Diluent NS.

  The PDGFR beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

• Interpolated concentrations of native PDGFR beta in mouse serum, plasma (EDTA), and cell culture supernatant samples.

  The concentrations of PDGFR beta were measured in duplicates, interpolated from the PDGFR beta standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, NIH/3T3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGFR beta concentration was determined to be 28.59 ng/mL in neat serum, 22.71 ng/mL in neat plasma (EDTA), and 0.578 ng/mL in neat NIH/3T3 cell culture supernatant.

• Sandwich ELISA - Mouse PDGFR beta ELISA Kit (ab253225)

  To learn more about the advantages of recombinant antibodies see here.

• Protocol Booklet

Click here to view the general protocols

• SDS download

  Country/Region

  Language

  

• Datasheet download

  Download

ab253225 has not yet been referenced specifically in any publications.