Human Angiopoietin 2/ANG2 ELISA Kit (ab316914) (2024)

Overview

• Product name

  Human Angiopoietin 2/ANG2 ELISA Kit
  See all Angiopoietin 2/ANG2 kits

• Detection method

  Colorimetric

• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Serum	8			5.2%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Serum	3			5%

• Sample type

  Cell culture supernatant, Saliva, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  See Also

  Human CD16 ELISA Kit- Extracellular (ab316909)Human IgA ELISA Kit (ab137980)Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (ab272199)Human IL-22(Interleukin 22) ELISA Kit-Elabscience

• Assay type

  Sandwich (quantitative)

• Sensitivity

  73 pg/ml

• Range

  125 pg/ml - 8000 pg/ml

• Recovery

  96 %
  

  Sample specific recovery

  Sample type	Average %	Range
  Cell culture supernatant	90	85% - 97%
  Saliva	92	89% - 95%
  Serum	96	80% - 112%
  Hep Plasma	83	65% - 99%
  EDTA Plasma	85	69% - 94%
  Cit plasma	90	80% - 107%

• Assay time

  1h 30m

• Assay duration

  One step assay

• Species reactivity

  Reacts with: Human
  

• Product overview

  Human Angiopoietin 2/ANG2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiopoietin 2/ANG2 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Saliva, Cell culture supernatant. QuantitateHuman Angiopoietin 2/ANG2 with 73 pg/ml sensitivity.

  
  

  SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  
  

  -Single-wash protocol reduces assay time to 90 minutes or less
  -High sensitivity, specificity and reproducibility from superior antibodies
  -Fully validated in biological samples
  -96-wells plate breakable into 12 x 8 wells strips

  
  

  A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

• Platform

  Pre-coated microplate (12 x 8 well strips)

Properties

• Storage instructions

  Store at +4°C. Please refer to protocols.

• Components	1 x 96 tests
  10X Human Angiopoietin 2/ANG2 Capture Antibody	1 x 600µl
  10X Human Angiopoietin 2/ANG2 Detector Antibody	1 x 600µl
  10X Wash Buffer PT (ab206977)	1 x 20ml
  Antibody Diluent 5BI	1 x 6ml
  Human Angiopoietin 2/ANG2 Lyophilized Recombinant Protein	2 vials
  Plate Seal	1 unit
  Sample Diluent NS (ab193972)	1 x 50ml
  SimpleStep Pre-Coated 96-Well Microplate (ab206978)	1 unit
  Stop Solution	1 x 12ml
  TMBDevelopment Solution	1 x 12ml

• Function

  Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

• Sequence similarities

  Contains 1 fibrinogen C-terminal domain.

• Domain

  The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.

• Cellular localization

  Secreted.

• Information by UniProt

• Alternative names

  • AGPT 2
  • Agpt2
  • ANG 2

  see all

• Database links

  • Entrez Gene: 285 Human
  • Omim: 601922 Human
  • SwissProt: O15123 Human
  • Unigene: 583870 Human

• Example of human Angiopoietin 2/ANG2 standard curve in Sample Diluent NS.

  Example of human Angiopoietin 2/ANG2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

• Interpolated concentrations of human Angiopoietin 2/ANG2 in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and HUVEC cell culture supernatant.

  Interpolated concentration of native Angiopoietin 2/ANG2 was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), plasma (heparin), and HUVEC cell culture supernatant. The interpolated dilution factor corrected values to neat sample are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

• Interpolated concentrations of Angiopoietin 2/ANG2 in normal human serum donors.

  Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin 2/ANG2 concentration was determined to be 2,168 pg/mL with a range of 1,269 – 3,011 pg/mL.

• Human Angiopoietin 2/ANG2 ELISA Kit

• Human Angiopoietin 2/ANG2 ELISA Kit

• Human Angiopoietin 2/ANG2 ELISA Kit

• Protocol Booklet

Click here to view the general protocols

• SDS download

  Country/Region

  Language

  

• Datasheet download

  Download

ab316914 has not yet been referenced specifically in any publications.