Human IgA ELISA Kit (ab137980) (2024)

Overview

• Product name

  Human IgA ELISA Kit
  See all IgA kits

• Detection method

  Colorimetric

  See Also

  Human CD16 ELISA Kit- Extracellular (ab316909)Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (ab272199)Human IL-22(Interleukin 22) ELISA Kit-ElabscienceHuman Angiopoietin 2/ANG2 ELISA Kit (ab316914)

• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Overall				5%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Overall				9.4%

• Sample type

  Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma

• Assay type

  Sandwich (quantitative)

• Sensitivity

  0.35 ng/ml

• Range

  1.56 ng/ml - 100 ng/ml

• Recovery

  97 %
  

• Assay time

  4h 0m

• Assay duration

  Multiple steps standard assay

• Species reactivity

  Reacts with: Human
  

• Product overview

  Human IgAin vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgA in plasma, serum, urine, saliva, milk, cerebrospinal fluid and cell culture supernatants.

  
  

  An IgA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IgA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IgA captured in plate.

  
  

  Get higher sensitivity in only 90 minutes with Human IgA ELISA Kit (ab196263) from our SimpleStep ELISA^® range.

  
  

  The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

• Platform

  Microplate

Properties

• Storage instructions

  Store at -20°C. Please refer to protocols.

• Components	1 x 96 tests
  100X Streptavidin-Peroxidase Conjugate	1 x 80µl
  10X Diluent N Concentrate	1 x 30ml
  20X Wash Buffer Concentrate	2 x 30ml
  50X Biotinylated Human IgA Antibody	1 x 120µl
  Chromogen Substrate	1 x 7ml
  IgA Microplate (12 x 8 well strips)	1 unit
  IgA Standard (Lyophilized)	1 vial
  Sealing Tapes	3 units
  Stop Solution	1 x 11ml

• Research areas

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Immunoglobulins ELISA kits

  See Also

  Human CA125 ELISA Kit (MUC16) (ab274402)

• Relevance

  Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.

• Alternative names

  • Hepatocellular carcinoma-associated protein TB6
  • Ig alpha 1 chain C region
  • Ig alpha 2 chain C region

  see all

• Database links

  • Entrez Gene: 5284 Human
  • Omim: 146900 Human
  • Omim: 147000 Human
  • Omim: 173880 Human
  • SwissProt: P01876 Human
  • SwissProt: P01877 Human

• Sandwich ELISA - IgA Human ELISA Kit (ab137980)

  IgA measured in biological fluids, background signal subtracted (duplicates +/- SD).

• Typical Standard Curve

  Representative Standard Curve using ab137980

• Protocol Booklet

Click here to view the general protocols

• SDS download

  Country/Region

  Language

  

• Datasheet download

  Download

ab137980 has been referenced in 2 publications.

• Rojas OL et al. Recirculating Intestinal IgA-Producing Cells Regulate Neuroinflammation via IL-10. Cell 176:610-624.e18 (2019). PubMed: 30612739
• Li H et al. Tripterygium Wilfordii inhibits tonsillar IgA production by downregulating IgA class switching in IgA nephropathy. Oncotarget 8:109027-109042 (2017). PubMed: 29312588