Human CA125 ELISA Kit (MUC16) (ab274402) (2024)

Overview

• Product name

  Human CA125 ELISA Kit (MUC16)
  See all MUC16 kits

• Detection method

  Colorimetric

• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Supernatant	8			5.1%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Supernatant	4			7.8%

• Sample type

  Cell culture supernatant, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma

• Assay type

  Sandwich (quantitative)

• Sensitivity

  0.02 U/ml

• Range

  0.09 U/ml - 6 U/ml

• Recovery

  Sample specific recovery

  Sample type	Average %	Range
  Cell culture supernatant	110	105% - 119%
  Milk	94	92% - 97%
  Urine	91	90% - 91%
  Serum	104	103% - 105%
  Hep Plasma	103	100% - 107%
  EDTA Plasma	103	100% - 107%
  Cit plasma	94	92% - 97%

  See Also

  Human CD16 ELISA Kit- Extracellular (ab316909)Human IgA ELISA Kit (ab137980)Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (ab272199)Human IL-22(Interleukin 22) ELISA Kit-Elabscience

• Assay time

  1h 30m

• Assay duration

  One step assay

• Species reactivity

  Reacts with: Human
  

• Product overview

  Human CA125 ELISA kit (ab274402) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CA125 protein in serum, plasma, urine, cell culture supernatant, and milk. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CA125 with 0.02 U/mL sensitivity.

  
  

  SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  
  

  -Single-wash protocol reduces assay time to 90 minutes or less

  
  

  -High sensitivity, specificity and reproducibility from superior antibodies

  
  

  -Fully validated in biological samples

  
  

  -96-wells plate breakable into 12 x 8 wells strips

  
  

  A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

• Notes

  Cancer antigen 125 (CA125, also known as Mucin 16) is a membrane associated cell surface protein containing approximately 22,096 amino acids. CA125 is the largest member of the Mucin family of proteins and is highly O-glycosylated. The hydrophilic nature of mucin proteins gives them the ability to form protective/lubricating gel-like barriers to protect against chemotherapy agents, foreign particles and infectious agents. CA125 is used as a biomarker for ovarian cancer detection, with about 90% of women with advanced ovarian cancer having elevated levels of CA125 present in their blood serum.

• Platform

  Pre-coated microplate (12 x 8 well strips)

Properties

• Storage instructions

  Store at +4°C. Please refer to protocols.

• Components	1 x 96 tests	10 x 96 tests
  10X Human CA125 (MUC16) Capture Antibody	1 x 600µl	1 x 6000µl
  10X Human CA125 (MUC16) Detector Antibody	1 x 600µl	1 x 6000µl
  10X Wash Buffer PT (ab206977)	1 x 20ml	1 x 200ml
  Antibody Diluent 5BI	1 x 6ml	10 x 6ml
  Human CA125 (MUC16) Lyophilized Recombinant Protein	2 vials	2 x 10 vials
  Plate Seals	1 unit	1 x 10 units
  Sample Diluent NS (ab193972)	1 x 50ml	2 x 250ml
  SimpleStep Pre-Coated 96-Well Microplate (ab206978)	1 unit	1 x 10 units
  Stop Solution	1 x 12ml	1 x 120ml
  TMBDevelopment Solution	1 x 12ml	1 x 120ml

• Research areas

  • Cell Type Markers
  • Tumor Associated

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • MUC

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • MUC

• Function

  Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

• Tissue specificity

  Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.

• Sequence similarities

  Contains 2 ANK repeats.
  Contains 56 SEA domains.

• Domain

  Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.

• Post-translational
  modifications

  Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
  Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
  May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
  May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

• Cellular localization

  Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.

• Information by UniProt

• Alternative names

  • CA 125
  • CA-125
  • CA125

  see all

• Database links

  • Entrez Gene: 94025 Human
  • Omim: 606154 Human
  • SwissProt: Q8WXI7 Human
  • Unigene: 432676 Human

• SimpleStep ELISA Protocol Diagram

  SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

• Human CA125 competitor curve comparison

  Standard Curve comparison between Human CA125 SimpleStep ELISA kit and traditional ELISA kit from leading competitor.

• Human CA125 standard curve comparison

  Standard Curve comparison between the original Human CA125 SimpleStep ELISA kit (ab195213) and current Human CA125 SimpleStep ELISA kit (ab274402).

• Example of human CA125 standard curve in Sample Diluent NS.

  The CA125 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

• Interpolated concentrations of native CA125 in Hela cell culture supernatant and milk samples.

  The concentrations of CA125 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: Hela cell culture supernatant, 50%, milk, 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 3 U/mL in Hela cell culture supernatant, 4.5 U/mL in milk.

• Serum from eight individual healthy CA125 female donors was measured in duplicate.

  Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CA125 concentration was determined to be 0.2 U/mL, 6 donors CA125 were below detection level.

• Sandwich ELISA - Human CA125 ELISA Kit(MUC16) (ab274402)

  To learn more about the advantages of recombinant antibodies see here.

• Protocol Booklet

Click here to view the general protocols

• SDS download

  Country/Region

  Language

  

• Datasheet download

  Download

ab274402 has been referenced in 1 publication.

• Kang C et al. Construction and Validation of a Lung Cancer Diagnostic Model Based on 6-Gene Methylation Frequency in Blood, Clinical Features, and Serum Tumor Markers. Comput Math Methods Med 2021:9987067 (2021). PubMed: 34257703