Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (ab272198) (2024)

Overview

• Product name

  Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP)
  See all PICP kits

• Detection method

  Colorimetric

• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Serum	8			3.1%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Serum	3			7.2%

• Sample type

  Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

• Assay type

  Sandwich (quantitative)

• Sensitivity

  8.15 pg/ml

• Range

  23.44 pg/ml - 1500 pg/ml

  See Also

  Human CD16 ELISA Kit- Extracellular (ab316909)Human IgA ELISA Kit (ab137980)Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (ab272199)Human IL-22(Interleukin 22) ELISA Kit-Elabscience

• Recovery

  Sample specific recovery

  Sample type	Average %	Range
  Serum	102	92% - 109%
  Cell culture media	107	103% - 109%
  Hep Plasma	89	85% - 92%
  EDTA Plasma	89	85% - 100%
  Cit plasma	90	80% - 106%

• Assay time

  1h 30m

• Assay duration

  One step assay

• Species reactivity

  Reacts with: Human
  

• Product overview

  Human Pro-Collagen I C-Terminal Propeptide ELISA kit (PICP) (ab272198) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pro-Collagen I C-Terminal Propeptide protein in serum, plasma and urine. It uses our proprietary SimpleStep ELISA® technology. QuantitateHumanPICP with 8.15 pg/mL sensitivity.

  
  

  SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  
  

  -Single-wash protocol reduces assay time to 90 minutes or less

  
  

  -High sensitivity, specificity and reproducibility from superior antibodies

  
  

  -Fully validated in biological samples

  
  

  -96-wells plate breakable into 12 x 8 wells strips

  
  

  A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

• Notes

  Pro-Collagen I C-Terminal Propeptide (PICP), encoded by the COL1A1 gene, is the fibrillar collagen that serves as a major component of type I collagen found in cartilage and most connective tissues. Studies have shown that mutations in the mutations in the Pro-Collagen I C-Terminal Propeptide are associated with osteoporosis, osteogenesis imperfecta diseases, and Ehlers-Danlos syndrome. The standard protein in this product is the C-terminal Propeptide region of Collagen alpha-1(I).

  Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
  It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

• Platform

  Pre-coated microplate (12 x 8 well strips)

Properties

• Storage instructions

  Store at +4°C. Please refer to protocols.

• Components	1 x 96 tests	1 x 96 tests
  10X Human Pro-Collagen I C-Terminal Propeptide (PICP) Capture Antibody	1 x 600µl	1 x 600µl
  10X Human Pro-Collagen I C-Terminal Propeptide (PICP) Detector Antibody	1 x 600µl	1 x 600µl
  10X Wash Buffer PT (ab206977)	1 x 20ml	1 x 20ml
  5X Cell Extraction Buffer PTR (ab193970)	1 x 10ml	1 x 10ml
  Antibody Diluent CPI2	1 x 6ml	1 x 6ml
  Human Pro-Collagen I C-Terminal Propeptide (PICP) Lyophilized Recombinant Protein	2 vials	2 vials
  Plate Seals	1 unit	1 unit
  Sample Diluent NS (ab193972)	1 x 50ml	1 x 50ml
  SimpleStep Pre-Coated 96-Well Microplate (ab206978)	1 unit	1 unit
  Stop Solution	1 x 12ml	1 x 12ml
  TMBDevelopment Solution	1 x 12ml	1 x 12ml

• Research areas

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

• Function

  Type I collagen is a member of group I collagen (fibrillar forming collagen).

• Tissue specificity

  Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

• Involvement in disease

  Caffey disease
  Ehlers-Danlos syndrome 1
  Ehlers-Danlos syndrome 7A
  Osteogenesis imperfecta 1
  Osteogenesis imperfecta 2
  Osteogenesis imperfecta 3
  Osteogenesis imperfecta 4
  Osteoporosis
  A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.

• Sequence similarities

  Belongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.

• Domain

  The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.

• Post-translational
  modifications

  Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

• Cellular localization

  Secreted > extracellular space > extracellular matrix.

  See Also

  Human Angiopoietin 2/ANG2 ELISA Kit (ab316914)

• Information by UniProt

• Alternative names

  • COL1A1
  • Alpha 1 type I collagen
  • Alpha-1 type I collagen

  see all

• Database links

  • Entrez Gene: 1277 Human
  • Omim: 120150 Human
  • SwissProt: P02452 Human
  • Unigene: 172928 Human
  • Unigene: 681002 Human

• SimpleStep ELISA Protocol Diagram

• Example of human Pro-Collagen I C-Terminal Propeptide standard curve in Sample Diluent NS.

• Interpolated concentrations of native Pro-Collagen I C-Terminal Propeptide in human serum and plasma samples.

• Interpolated concentrations of native Pro-Collagen I C-Terminal Propeptide in human skin tissue extract sample based on a 50 µg/mL extract load.

• Serum from ten individual healthy human female donors was measured in duplicate.

• Sandwich ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (ab272198)

  To learn more about the advantages of recombinant antibodies see here.

• Protocol Booklet

Click here to view the general protocols

• SDS download

  Country/Region

  Language

  

• Datasheet download

  Download

ab272198 has not yet been referenced specifically in any publications.